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a mouse monoclonal primary antibody against collagen type ii alpha 1  (Millipore)


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    Millipore a mouse monoclonal primary antibody against collagen type ii alpha 1
    Collagen Type II <t>alpha</t> <t>1</t> IHC staining of the NP at each time point (original magnification, ×400). (a) Fresh tissue; (b, d, and f) tissue collagen Type II alpha 1 staining of organ controls at days 3, 7, and 14, respectively; (c, e, and g) collagen Type II alpha 1 staining of tissues under static load at days 3, 7, and 14, respectively. The intensity of staining remained constant by 14 days in the controls, and no significant difference was observed between the controls and fresh tissue (a, arrow). The samples under static load showed a significant enhancement in IHC intensity for the initial 3 days (c, arrowheads); however, the staining was obviously reduced after 3 days, by 14 days (g), the staining was significantly decreased (arrow) and significantly different from the controls. IHC: Immunohistochemistry; NP: Nucleus pulposus.
    A Mouse Monoclonal Primary Antibody Against Collagen Type Ii Alpha 1, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/a+mouse+monoclonal+primary+antibody+against+collagen+type+ii+alpha+1/a+mouse+monoclonal+primary+antibody+against+collagen+type+ii+alpha+1/pmc05040021-95-7-17
    Average 90 stars, based on 1 article reviews
    a mouse monoclonal primary antibody against collagen type ii alpha 1 - by Bioz Stars, 2026-11
    90/100 stars

    Images

    1) Product Images from "Effect of Static Load on the Nucleus Pulposus of Rabbit Intervertebral Disc Motion Segment in Ex vivo Organ Culture"

    Article Title: Effect of Static Load on the Nucleus Pulposus of Rabbit Intervertebral Disc Motion Segment in Ex vivo Organ Culture

    Journal: Chinese Medical Journal

    doi: 10.4103/0366-6999.190666

    Collagen Type II alpha 1 IHC staining of the NP at each time point (original magnification, ×400). (a) Fresh tissue; (b, d, and f) tissue collagen Type II alpha 1 staining of organ controls at days 3, 7, and 14, respectively; (c, e, and g) collagen Type II alpha 1 staining of tissues under static load at days 3, 7, and 14, respectively. The intensity of staining remained constant by 14 days in the controls, and no significant difference was observed between the controls and fresh tissue (a, arrow). The samples under static load showed a significant enhancement in IHC intensity for the initial 3 days (c, arrowheads); however, the staining was obviously reduced after 3 days, by 14 days (g), the staining was significantly decreased (arrow) and significantly different from the controls. IHC: Immunohistochemistry; NP: Nucleus pulposus.
    Figure Legend Snippet: Collagen Type II alpha 1 IHC staining of the NP at each time point (original magnification, ×400). (a) Fresh tissue; (b, d, and f) tissue collagen Type II alpha 1 staining of organ controls at days 3, 7, and 14, respectively; (c, e, and g) collagen Type II alpha 1 staining of tissues under static load at days 3, 7, and 14, respectively. The intensity of staining remained constant by 14 days in the controls, and no significant difference was observed between the controls and fresh tissue (a, arrow). The samples under static load showed a significant enhancement in IHC intensity for the initial 3 days (c, arrowheads); however, the staining was obviously reduced after 3 days, by 14 days (g), the staining was significantly decreased (arrow) and significantly different from the controls. IHC: Immunohistochemistry; NP: Nucleus pulposus.

    Techniques Used: Immunohistochemistry, Staining

    Collagen Type II alpha 1 IHC staining intensity of the NP at each time point. No significant differences in collagen Type II alpha 1 staining intensity were observed between the fresh tissues and controls. The samples under static load showed a significant enhancement at 3 day, and significant differences were observed between the controls and fresh tissues. At 7 day, the staining was obviously decreased, and by 14 days, it was significantly decreased ( P < 0.05) and significantly different from that of the controls. The values represent the mean ± SD. * P < 0.05 versus day 0 of the same group; † P < 0.05 versus day 3 of the same group; ‡ P < 0.05 versus controls. IHC: Immunohistochemistry; NP: Nucleus pulposus; SD: Standard deviation.
    Figure Legend Snippet: Collagen Type II alpha 1 IHC staining intensity of the NP at each time point. No significant differences in collagen Type II alpha 1 staining intensity were observed between the fresh tissues and controls. The samples under static load showed a significant enhancement at 3 day, and significant differences were observed between the controls and fresh tissues. At 7 day, the staining was obviously decreased, and by 14 days, it was significantly decreased ( P < 0.05) and significantly different from that of the controls. The values represent the mean ± SD. * P < 0.05 versus day 0 of the same group; † P < 0.05 versus day 3 of the same group; ‡ P < 0.05 versus controls. IHC: Immunohistochemistry; NP: Nucleus pulposus; SD: Standard deviation.

    Techniques Used: Immunohistochemistry, Staining, Standard Deviation

    Collagen Type II alpha 1 IHC staining intensity of the NP during organ culture
    Figure Legend Snippet: Collagen Type II alpha 1 IHC staining intensity of the NP during organ culture

    Techniques Used: Immunohistochemistry

    Relative expression quantified by real-time PCR. An obvious downregulation of Agg was observed in the controls after 3 days whereas a significant downregulation was observed under static load during culture compared with fresh tissue and controls. An upregulation of COL2A1 was observed in response to static load by 3 days, and the expression of this gene subsequently decreased until day 14 to below detectable levels. COL2A1 was downregulated in the controls by 3 days whereas no significant downregulation was observed after culturing, and significant differences were observed between the two groups after culture. The values represent the means ± SD normalized to fresh tissue. * P < 0.05 versus day 0 of the same group; † P < 0.05 versus day 3 of the same group; ‡ P < 0.05 versus controls. PCR: Polymerase chain reaction; Agg : Aggrecan; COL2A1 : Collagen Type II alpha 1; SD: Standard deviation.
    Figure Legend Snippet: Relative expression quantified by real-time PCR. An obvious downregulation of Agg was observed in the controls after 3 days whereas a significant downregulation was observed under static load during culture compared with fresh tissue and controls. An upregulation of COL2A1 was observed in response to static load by 3 days, and the expression of this gene subsequently decreased until day 14 to below detectable levels. COL2A1 was downregulated in the controls by 3 days whereas no significant downregulation was observed after culturing, and significant differences were observed between the two groups after culture. The values represent the means ± SD normalized to fresh tissue. * P < 0.05 versus day 0 of the same group; † P < 0.05 versus day 3 of the same group; ‡ P < 0.05 versus controls. PCR: Polymerase chain reaction; Agg : Aggrecan; COL2A1 : Collagen Type II alpha 1; SD: Standard deviation.

    Techniques Used: Expressing, Real-time Polymerase Chain Reaction, Polymerase Chain Reaction, Standard Deviation

    Related Articles

    Incubation:

    Article Title: Effect of Static Load on the Nucleus Pulposus of Rabbit Intervertebral Disc Motion Segment in Ex vivo Organ Culture
    Article Snippet: .. Then, the sections were incubated with a mouse monoclonal primary antibody against collagen Type II alpha 1 (Sigma-Aldrich, Bale, Switzerland) diluted 1:20 overnight at 4°C. .. Negative controls were used for which the primary antibody was replaced with an equal concentration of mouse IgG (Sigma).



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    a mouse monoclonal primary antibody against collagen type ii alpha 1  (Millipore)
    90
    Millipore a mouse monoclonal primary antibody against collagen type ii alpha 1
    Collagen Type II <t>alpha</t> <t>1</t> IHC staining of the NP at each time point (original magnification, ×400). (a) Fresh tissue; (b, d, and f) tissue collagen Type II alpha 1 staining of organ controls at days 3, 7, and 14, respectively; (c, e, and g) collagen Type II alpha 1 staining of tissues under static load at days 3, 7, and 14, respectively. The intensity of staining remained constant by 14 days in the controls, and no significant difference was observed between the controls and fresh tissue (a, arrow). The samples under static load showed a significant enhancement in IHC intensity for the initial 3 days (c, arrowheads); however, the staining was obviously reduced after 3 days, by 14 days (g), the staining was significantly decreased (arrow) and significantly different from the controls. IHC: Immunohistochemistry; NP: Nucleus pulposus.
    A Mouse Monoclonal Primary Antibody Against Collagen Type Ii Alpha 1, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/a+mouse+monoclonal+primary+antibody+against+collagen+type+ii+alpha+1/a+mouse+monoclonal+primary+antibody+against+collagen+type+ii+alpha+1/pmc05040021-95-7-17
    Average 90 stars, based on 1 article reviews
    a mouse monoclonal primary antibody against collagen type ii alpha 1 - by Bioz Stars, 2026-11
    90/100 stars
    		  Buy from Supplier

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    Collagen Type II alpha 1 IHC staining of the NP at each time point (original magnification, ×400). (a) Fresh tissue; (b, d, and f) tissue collagen Type II alpha 1 staining of organ controls at days 3, 7, and 14, respectively; (c, e, and g) collagen Type II alpha 1 staining of tissues under static load at days 3, 7, and 14, respectively. The intensity of staining remained constant by 14 days in the controls, and no significant difference was observed between the controls and fresh tissue (a, arrow). The samples under static load showed a significant enhancement in IHC intensity for the initial 3 days (c, arrowheads); however, the staining was obviously reduced after 3 days, by 14 days (g), the staining was significantly decreased (arrow) and significantly different from the controls. IHC: Immunohistochemistry; NP: Nucleus pulposus.

    Journal: Chinese Medical Journal

    Article Title: Effect of Static Load on the Nucleus Pulposus of Rabbit Intervertebral Disc Motion Segment in Ex vivo Organ Culture

    doi: 10.4103/0366-6999.190666

    Figure Lengend Snippet: Collagen Type II alpha 1 IHC staining of the NP at each time point (original magnification, ×400). (a) Fresh tissue; (b, d, and f) tissue collagen Type II alpha 1 staining of organ controls at days 3, 7, and 14, respectively; (c, e, and g) collagen Type II alpha 1 staining of tissues under static load at days 3, 7, and 14, respectively. The intensity of staining remained constant by 14 days in the controls, and no significant difference was observed between the controls and fresh tissue (a, arrow). The samples under static load showed a significant enhancement in IHC intensity for the initial 3 days (c, arrowheads); however, the staining was obviously reduced after 3 days, by 14 days (g), the staining was significantly decreased (arrow) and significantly different from the controls. IHC: Immunohistochemistry; NP: Nucleus pulposus.

    Article Snippet: Then, the sections were incubated with a mouse monoclonal primary antibody against collagen Type II alpha 1 (Sigma-Aldrich, Bale, Switzerland) diluted 1:20 overnight at 4°C.

    Techniques: Immunohistochemistry, Staining

    Collagen Type II alpha 1 IHC staining intensity of the NP at each time point. No significant differences in collagen Type II alpha 1 staining intensity were observed between the fresh tissues and controls. The samples under static load showed a significant enhancement at 3 day, and significant differences were observed between the controls and fresh tissues. At 7 day, the staining was obviously decreased, and by 14 days, it was significantly decreased ( P < 0.05) and significantly different from that of the controls. The values represent the mean ± SD. * P < 0.05 versus day 0 of the same group; † P < 0.05 versus day 3 of the same group; ‡ P < 0.05 versus controls. IHC: Immunohistochemistry; NP: Nucleus pulposus; SD: Standard deviation.

    Journal: Chinese Medical Journal

    Article Title: Effect of Static Load on the Nucleus Pulposus of Rabbit Intervertebral Disc Motion Segment in Ex vivo Organ Culture

    doi: 10.4103/0366-6999.190666

    Figure Lengend Snippet: Collagen Type II alpha 1 IHC staining intensity of the NP at each time point. No significant differences in collagen Type II alpha 1 staining intensity were observed between the fresh tissues and controls. The samples under static load showed a significant enhancement at 3 day, and significant differences were observed between the controls and fresh tissues. At 7 day, the staining was obviously decreased, and by 14 days, it was significantly decreased ( P < 0.05) and significantly different from that of the controls. The values represent the mean ± SD. * P < 0.05 versus day 0 of the same group; † P < 0.05 versus day 3 of the same group; ‡ P < 0.05 versus controls. IHC: Immunohistochemistry; NP: Nucleus pulposus; SD: Standard deviation.

    Article Snippet: Then, the sections were incubated with a mouse monoclonal primary antibody against collagen Type II alpha 1 (Sigma-Aldrich, Bale, Switzerland) diluted 1:20 overnight at 4°C.

    Techniques: Immunohistochemistry, Staining, Standard Deviation

    Collagen Type II alpha 1 IHC staining intensity of the NP during organ culture

    Journal: Chinese Medical Journal

    Article Title: Effect of Static Load on the Nucleus Pulposus of Rabbit Intervertebral Disc Motion Segment in Ex vivo Organ Culture

    doi: 10.4103/0366-6999.190666

    Figure Lengend Snippet: Collagen Type II alpha 1 IHC staining intensity of the NP during organ culture

    Article Snippet: Then, the sections were incubated with a mouse monoclonal primary antibody against collagen Type II alpha 1 (Sigma-Aldrich, Bale, Switzerland) diluted 1:20 overnight at 4°C.

    Techniques: Immunohistochemistry

    Relative expression quantified by real-time PCR. An obvious downregulation of Agg was observed in the controls after 3 days whereas a significant downregulation was observed under static load during culture compared with fresh tissue and controls. An upregulation of COL2A1 was observed in response to static load by 3 days, and the expression of this gene subsequently decreased until day 14 to below detectable levels. COL2A1 was downregulated in the controls by 3 days whereas no significant downregulation was observed after culturing, and significant differences were observed between the two groups after culture. The values represent the means ± SD normalized to fresh tissue. * P < 0.05 versus day 0 of the same group; † P < 0.05 versus day 3 of the same group; ‡ P < 0.05 versus controls. PCR: Polymerase chain reaction; Agg : Aggrecan; COL2A1 : Collagen Type II alpha 1; SD: Standard deviation.

    Journal: Chinese Medical Journal

    Article Title: Effect of Static Load on the Nucleus Pulposus of Rabbit Intervertebral Disc Motion Segment in Ex vivo Organ Culture

    doi: 10.4103/0366-6999.190666

    Figure Lengend Snippet: Relative expression quantified by real-time PCR. An obvious downregulation of Agg was observed in the controls after 3 days whereas a significant downregulation was observed under static load during culture compared with fresh tissue and controls. An upregulation of COL2A1 was observed in response to static load by 3 days, and the expression of this gene subsequently decreased until day 14 to below detectable levels. COL2A1 was downregulated in the controls by 3 days whereas no significant downregulation was observed after culturing, and significant differences were observed between the two groups after culture. The values represent the means ± SD normalized to fresh tissue. * P < 0.05 versus day 0 of the same group; † P < 0.05 versus day 3 of the same group; ‡ P < 0.05 versus controls. PCR: Polymerase chain reaction; Agg : Aggrecan; COL2A1 : Collagen Type II alpha 1; SD: Standard deviation.

    Article Snippet: Then, the sections were incubated with a mouse monoclonal primary antibody against collagen Type II alpha 1 (Sigma-Aldrich, Bale, Switzerland) diluted 1:20 overnight at 4°C.

    Techniques: Expressing, Real-time Polymerase Chain Reaction, Polymerase Chain Reaction, Standard Deviation